A double beam spectrophotometer is an instrument used to measure the absorbance of a sample by comparing it with a reference (blank). It works by splitting a single light beam into two paths—one passes through the sample and the other through the reference—allowing simultaneous measurement. This comparison improves accuracy and reduces errors caused by fluctuations in light intensity. It is widely used in chemical, pharmaceutical, and biological analysis for determining the concentration of substances.
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A double beam spectrophotometer is an instrument used to measure the absorbance of a sample by comparing it with a reference (blank). It works by splitting a single light beam into two paths—one passes through the sample and the other through the reference—allowing simultaneous measurement. This comparison improves accuracy and reduces errors caused by fluctuations in light intensity. It is widely used in chemical, pharmaceutical, and biological analysis for determining the concentration of substances.
The BV-DB5WL Double Beam UV-Vis Spectrophotometer is a versatile instrument for laboratories and industrial applications. It supports photometric analysis, kinetics, spectrum scanning, DNA/protein testing, multi-wavelength measurements, as well as applications in drug formulation, environmental monitoring, and toxicology studies.
| Specification | Details |
|---|---|
| Wavelength Range | 190 to 1100 nm |
| Spectral Bandwidth | 0.5, 1, 2, 4 and 5 nm |
| Wavelength Setting | Automatic |
| Optical System | Double Beam, Grating 1200 lines/ mm |
| Wavelength Accuracy | ± 0.1nm at 656.1 nm, ± 0.3 nm at all |
| Wavelength Reproducibility | Up to 0.1 nm |
| Photometric Accuracy | ±0.2% T (0 to 100% T), ±0.002 A (0 to 0.5A), ±0.004 A (0.5 to 1A) |
| Photometric Repeatability | Up to 0.15% T (0 to 100% T), 0.001 A (0 to 0.5 A), 0.002 A(0.5 to 1 A) |
| Photometric Range | 0 to 200% T, -0.3 to 3 A, 0 to 9999 C |
| Stray Light Up to | 0.03% T at 220 nm, 360 nm |
| Stability | ± 0.0003 A/h at 500 nm |
| Baseline Flatness | ±0.0010 A |
| Noise | 0.0005 A at 500 nm |
| Work Mode | T, A, C, E |
| Scanning Speed | Hi, Med, Low (Max. 3000 nm/min) |
| Display | 7-inches Colour Screen |
| Light Source | Deuterium and tungsten lamp |
| Detector | Silicon Photodiode |
| Cuvette Holder | 10 mm manual 4-cell holder |
| Output | USB drive, USB host, RS232 |
| Power Supply | AC 110 to 220V 50 to 60Hz |
| Unit dimension | 595 x 480 x 250 mm |
| Dimensions | 885 × 695 × 525 mm |
| Net weight | 30 kg |
| Gross Weight | 47 kg |